Methyl Jasmonate Elicited Helichrysum stoechas (L.) Moench Cell Suspensions, A Promising Source of Extracts with Allelopathic Activity?

Helichrysum stoechas (L.) Moench cell suspensions were analyzed by LC-ESI-QTOF, which highlighted the predominance of 3,5-O-dicaffeoylquinic acid (3,5-diCQA). Elicitation H. cells with methyl jasmonate (200 mM) led to a massive rise in 3,5-diCQA, up 5-fold-increase compared control, reaching concentration 10.2 mg. g-1dry weight after 14 days culture. Previous data showed that diCQA isomers are potent allelopathic compounds, thus methanolic extracts control and MeJa-elicited tested for their phytotoxicity. With this mind, activity on seed germination seedling growth model plant Lepidium sativum tested. Phytotoxicity both occurred dose-dependent manner, greater elicited extract. Indeed, range from 0.31 0.83 mL-1, latter significantly higher inhibition rate L. seedlings root growth, when The presented may contribute explore new strategies towards conception bioherbicides origin. To our knowledge, study is first report use grown vitro as raw material production metabolites.